Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease

Summary: Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune signaling pathways triggered by nucleic acid...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Calvin Krollmann, Kevin Cieslak, Ruth-Miriam Koerber, Hella Luksch, Angela Rösen-Wolff, Peter Brossart, Lino L. Teichmann
Format:	Article
Language:	English
Published:	Elsevier 2022-06-01
Subjects:	Flow Cytometry/Mass Cytometry Immunology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722001897