Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota

Abstract Background Acquiring representative bacterial 16S rRNA gene community profiles in plant microbiome studies can be challenging due to the excessive co-amplification of host chloroplast and mitochondrial rRNA gene sequences that reduce counts of plant-associated bacterial sequences. Peptide N...

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Bibliographic Details

Published in:	Environmental Microbiome
Main Authors:	Usman Hussain, Jim Downie, Amy Ellison, Sandra Denman, James McDonald, Marine C. Cambon
Format:	Article
Language:	English
Published:	BMC 2025-01-01
Subjects:	Chloroplast Mitochondria Microbiome 16S rRNA gene sequencing PCR qPCR
Online Access:	https://doi.org/10.1186/s40793-025-00674-w