Enhancement of Activity and Thermostability of Keratinase From Pseudomonas aeruginosa CCTCC AB2013184 by Directed Evolution With Noncanonical Amino Acids

A keratinase from Pseudomonas aeruginosa (KerPA), which belongs to the M4 family of metallopeptidases, was characterised in this study. This enzyme was engineered with non-canonical amino acids (ncAAs) using genetic code expansion. Several variants with enhanced activity and thermostability were ide...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Xianchao Pan, Jian Yang, Peijuan Xie, Jing Zhang, Famin Ke, Xiurong Guo, Manyu Liang, Li Liu, Qin Wang, Xiaowei Gao
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-10-01
Subjects:	metallopeptidase keratinase genetic code expansion noncanonical amino acid protein engineering directed evolution
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2021.770907/full