Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis

Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purif...

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Bibliographic Details

Published in:	Brazilian Journal of Medical and Biological Research
Main Authors:	N.S. Sato, T. Suzuki, T. Ueda, K. Watanabe, R.D.C. Hirata, M.H. Hirata
Format:	Article
Language:	English
Published:	Associação Brasileira de Divulgação Científica 2004-07-01
Subjects:	Treponema pallidum Cloning Recombinant antigen Syphilis GST
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700002