Accelerated reprogramming of hiPSCs into functional brain endothelial-like cells using multiplexed CRISPR activation

Abstract Human induced pluripotent stem cells (hiPSCs) offer a renewable and scalable source for generating brain endothelial cells (BECs), enabling the development of in vitro blood-brain barrier (BBB) models that closely reflect human physiology. In this study, we demonstrate a streamlined differe...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Roy W. Hwang, Yara Khalil, Ewa Baumann, Junzhuo Huang, Claudie Charlebois, Marina Rukhlova, Tyler M. Renner, Ziying Liu, Anna Jezierski, Mads Kærn, Will J. Costain
Format:	Article
Language:	English
Published:	Nature Portfolio 2026-04-01
Subjects:	CRISPR Brain endothelial cells Blood-brain barrier iPSC Transcriptional reprogramming Synthetic biology
Online Access:	https://doi.org/10.1038/s41598-026-46961-5