Covalent and Site-Specific Immobilization of a Fluorogenic Sensor Protein on Cellulose-Based Paper for Detection of Lactate in Cell Culture Media

Lactate is a key metabolite with applications ranging from monitoring training efficiency to early sepsis detection and monitoring the metabolic state of cell cultures. In this study, a paper-based lactate sensor utilizing a fluorescent readout was developed. Unlike common lactate dehydrogenase (LDH...

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Bibliographic Details

Published in:	Biosensors
Main Authors:	Ingo Bork, Viktoria Höfling, Janine Becker, Markus Biesalski, Tobias Meckel, Harald Kolmar
Format:	Article
Language:	English
Published:	MDPI AG 2025-09-01
Subjects:	lactate sensor fluorogenic sensor paper-based sensor covalent paper modification site-specific immobilization
Online Access:	https://www.mdpi.com/2079-6374/15/10/643