Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae in sheep

Abstract Background Mycoplasmal pneumonia is an important infectious disease that threatens sheep and goat production worldwide, and Mycoplasma ovipneumoniae is one of major etiological agent causing mycoplasmal pneumonia. Recombinase polymerase amplification (RPA) is an isothermal nucleic acid ampl...

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Bibliographic Details

Published in:	BMC Veterinary Research
Main Authors:	Jinfeng Wang, Ruiwen Li, Xiaoxia Sun, Libing Liu, Xuepiao Hao, Jianchang Wang, Wanzhe Yuan
Format:	Article
Language:	English
Published:	BMC 2020-06-01
Subjects:	Mycoplasma ovipneumoniae 16S rRNA gene Real-time RPA Lateral flow strip Isothermal amplification
Online Access:	http://link.springer.com/article/10.1186/s12917-020-02387-3