MDC-Analyzer: A novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites

Recent computational and bioinformatics advances have enabled the efficient creation of novel biocatalysts by reducing amino acid variability at hot spot regions. To further expand the utility of this strategy, we present here a tool called Multi-site Degenerate Codon Analyzer (MDC-Analyzer) for the...

Full description

Bibliographic Details

Published in:	BioTechniques
Main Authors:	Lixia Tang, Xiong Wang, Beibei Ru, Hengfei Sun, Jian Huang, Hui Gao
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2014-06-01
Subjects:	partial randomization intelligent library design contiguous sites data-driven protein engineering
Online Access:	https://www.future-science.com/doi/10.2144/000114177