Protocol to examine immune subpopulations in murine conjunctiva and lacrimal gland using flow cytometry

Summary: Here, we present a protocol for the examination of immune cells in the murine conjunctiva and lacrimal gland using flow cytometry. We describe steps for dissection, preparation of high-quality single-cell suspensions, utilization of comprehensive staining panels, and optimization of flow cy...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Baikai Ma, Yifan Zhou, Hongyu Duan, Zhengze Sun, Wenling Han, Hong Qi
Format:	Article
Language:	English
Published:	Elsevier 2024-03-01
Subjects:	Cell Biology Cell isolation Flow Cytometry Immunology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724000868