The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein
Low-temperature storage is a common strategy for preserving and transporting vegetables and fruits. However, many fruits are hypersensitive to chilling injury, including bananas. In the present study, storage conditions of 11 °C delayed the ripening of Fenjiao (<i>Musa</i> ABB P...
| Published in: | Biomolecules |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Online Access: | https://www.mdpi.com/2218-273X/9/10/552 |
| _version_ | 1852821309296214016 |
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| author | Zunyang Song Jiajia Qin Qiuli Zheng Xiaochun Ding Weixin Chen Wangjin Lu Xueping Li Xiaoyang Zhu |
| author_facet | Zunyang Song Jiajia Qin Qiuli Zheng Xiaochun Ding Weixin Chen Wangjin Lu Xueping Li Xiaoyang Zhu |
| author_sort | Zunyang Song |
| collection | DOAJ |
| container_title | Biomolecules |
| description | Low-temperature storage is a common strategy for preserving and transporting vegetables and fruits. However, many fruits are hypersensitive to chilling injury, including bananas. In the present study, storage conditions of 11 °C delayed the ripening of Fenjiao (<i>Musa</i> ABB Pisang Awak) banana, and the pulp could be softened after ethephon treatment. Storage conditions of 7 °C prevented fruit from fully softening, and fruit contained a significantly higher starch content and lower soluble sugar content. <i>MaEBF1</i>, a critical gene component in the ethylene signaling pathway, was repressed during ripening after fruit had been stored for 12 days at 7 °C. The expression of a series of starch degradation-related genes and a <i>MaNAC67-like</i> gene were also severely repressed. Both <i>MaEBF1</i> and <i>MaNAC67</i>-like genes were ethylene-inducible and localized in the nucleus. MaNAC67-like protein was able to physically bind to the promoter of genes associated with starch degradation, including <i>MaBAM6</i>, <i>MaSEX4</i>, and <i>MaMEX1</i>. Yeast two-hybrid, GST-pull down, and BiFC assays showed that MaEBF1 interacted with the MaNAC67-like protein, and their interaction further activated the promoters of <i>MaBAM6</i> and <i>MaSEX4</i>. The current study indicates that MaNAC67-like is a direct regulator of starch degradation and potential for involvement in regulating chilling-inhibited starch degradation by interacting with the ethylene signaling components in banana fruit. The present work paves the way for further functional analysis of <i>MaEBF1</i> and <i>MaNAC67-like</i> in banana, which will be useful for understanding the regulation of banana starch metabolism and fruit ripening. |
| format | Article |
| id | doaj-art-2cfcde4b588a4e60b708febee0accad6 |
| institution | Directory of Open Access Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-2cfcde4b588a4e60b708febee0accad62025-08-19T20:32:08ZengMDPI AGBiomolecules2218-273X2019-09-0191055210.3390/biom9100552biom9100552The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like ProteinZunyang Song0Jiajia Qin1Qiuli Zheng2Xiaochun Ding3Weixin Chen4Wangjin Lu5Xueping Li6Xiaoyang Zhu7State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaLow-temperature storage is a common strategy for preserving and transporting vegetables and fruits. However, many fruits are hypersensitive to chilling injury, including bananas. In the present study, storage conditions of 11 °C delayed the ripening of Fenjiao (<i>Musa</i> ABB Pisang Awak) banana, and the pulp could be softened after ethephon treatment. Storage conditions of 7 °C prevented fruit from fully softening, and fruit contained a significantly higher starch content and lower soluble sugar content. <i>MaEBF1</i>, a critical gene component in the ethylene signaling pathway, was repressed during ripening after fruit had been stored for 12 days at 7 °C. The expression of a series of starch degradation-related genes and a <i>MaNAC67-like</i> gene were also severely repressed. Both <i>MaEBF1</i> and <i>MaNAC67</i>-like genes were ethylene-inducible and localized in the nucleus. MaNAC67-like protein was able to physically bind to the promoter of genes associated with starch degradation, including <i>MaBAM6</i>, <i>MaSEX4</i>, and <i>MaMEX1</i>. Yeast two-hybrid, GST-pull down, and BiFC assays showed that MaEBF1 interacted with the MaNAC67-like protein, and their interaction further activated the promoters of <i>MaBAM6</i> and <i>MaSEX4</i>. The current study indicates that MaNAC67-like is a direct regulator of starch degradation and potential for involvement in regulating chilling-inhibited starch degradation by interacting with the ethylene signaling components in banana fruit. The present work paves the way for further functional analysis of <i>MaEBF1</i> and <i>MaNAC67-like</i> in banana, which will be useful for understanding the regulation of banana starch metabolism and fruit ripening.https://www.mdpi.com/2218-273X/9/10/552fenjiao bananaripening disorderstarch degradationchilling injurymaebf1manac67-like |
| spellingShingle | Zunyang Song Jiajia Qin Qiuli Zheng Xiaochun Ding Weixin Chen Wangjin Lu Xueping Li Xiaoyang Zhu The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein fenjiao banana ripening disorder starch degradation chilling injury maebf1 manac67-like |
| title | The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein |
| title_full | The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein |
| title_fullStr | The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein |
| title_full_unstemmed | The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein |
| title_short | The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein |
| title_sort | involvement of the banana f box protein maebf1 in regulating chilling inhibited starch degradation through interaction with a manac67 like protein |
| topic | fenjiao banana ripening disorder starch degradation chilling injury maebf1 manac67-like |
| url | https://www.mdpi.com/2218-273X/9/10/552 |
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