Addressing the dynamic nature of reference data: a new nucleotide database for robust metagenomic classification

ABSTRACT Accurate metagenomic classification relies on comprehensive, up-to-date, and validated reference databases. While the NCBI BLAST Nucleotide (nt) database, encompassing a vast collection of sequences from all domains of life, represents an invaluable resource, its massive size—currently exce...

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Bibliographic Details

Published in:	mSystems
Main Authors:	Jose Manuel Martí, Car Reen Kok, James B. Thissen, Nisha J. Mulakken, Aram Avila-Herrera, Crystal J. Jaing, Jonathan E. Allen, Nicholas A. Be
Format:	Article
Language:	English
Published:	American Society for Microbiology 2025-04-01
Subjects:	metagenomics taxonomic classification reference database NCBI BLAST nt Centrifuge Recentrifuge
Online Access:	https://journals.asm.org/doi/10.1128/msystems.01239-24