A Single Active-Site Mutagenesis Confers Enhanced Activity and/or Changed Product Distribution to a Pentalenene Synthase from <i>Streptomyces</i> sp. PSKA01

Pentalenene is a ternary cyclic sesquiterpene formed via the ionization and cyclization of farnesyl pyrophosphate (FPP), which is catalyzed by pentalenene synthase (PentS). To better understand the cyclization reactions, it is necessary to identify more key sites and elucidate their roles in terms o...

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Bibliographic Details

Published in:	Bioengineering
Main Authors:	Hongshuang Liu, Senbiao Fang, Lin Zhao, Xiao Men, Haibo Zhang
Format:	Article
Language:	English
Published:	MDPI AG 2023-03-01
Subjects:	pentalenene pentalenene synthase random mutagenesis site-saturation mutagenesis molecular docking molecular dynamics simulations
Online Access:	https://www.mdpi.com/2306-5354/10/3/392