A systematic comparison of CRISPR-Cas9 allele editing in Candida auris demonstrates unreliable cassette integration and effective episomal plasmid-based editing

Abstract Candidozyma (Candida) auris is an emergent fungal pathogen of significant interest for molecular research. A handful of CRISPR-Cas9 based allele editing tools have been optimized for C. auris. Nonetheless, allele editing in this species remains a significant challenge, and different systems...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Dimitrios Sofras, Hans Carolus, Ana Subotić, Celia Lobo Romero, Craig L. Ennis, Lize Cuypers, Katrien Lagrou, Aaron D. Hernday, Clarissa J. Nobile, Jeffrey M. Rybak, Patrick Van Dijck
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-10-01
Online Access:	https://doi.org/10.1038/s41598-025-19115-2