Comprehensive comparison of enzymatic and bisulfite DNA methylation analysis in clinically relevant samples

Abstract Background Bisulfite conversion is considered the gold standard for DNA methylation analysis, but it damages DNA and performs sub-optimally with clinical samples (e.g., formalin-fixed paraffin-embedded and circulating free plasma DNA (cfDNA)). Here we describe a comprehensive comparison of...

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Bibliographic Details

Published in:	Clinical Epigenetics
Main Authors:	Barrett Nuttall, Daniel L. Karl, Kathleen Burke, Megan Callahan, Kerrin Mendler, Pablo Cingolani, Steven Criscione, Serhiy Naumenko, Elena Bibikova, Veerendra Munugalavadla, John C. Byrd, Richard R. Furman, Jennifer R. Brown, Andrew Mortlock, Brian A. Dougherty, J. Carl Barrett, Maurizio Scaltriti, James Hadfield
Format:	Article
Language:	English
Published:	BMC 2025-10-01
Subjects:	Methylation Enzymatic methylation sequencing Bisulfite sequencing Targeted methylation sequencing Chronic lymphocytic leukemia
Online Access:	https://doi.org/10.1186/s13148-025-01959-0