Live-Cell Nucleocytoplasmic Protein Shuttle Assay Utilizing Laser Confocal Microscopy and FRAP

In eukaryotes, protein trafficking to and from the nucleus, or shuttling, has been demonstrated to be an important function for proteins that have vital roles in one or both subcellular compartments. Current techniques of detecting protein nuclear shuttling are extremely labor intensive and only sta...

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Bibliographic Details
Published in:BioTechniques
Main Authors: Jenny L. Howell, Ray Truant
Format: Article
Language:English
Published: Taylor & Francis Group 2002-01-01
Online Access:https://www.future-science.com/doi/10.2144/02321st04