Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic Mice

The introduction of megabase-sized large DNA fragments into the germline has been a difficult task. Although microcell-mediated chromosome transfer into mouse embryonic stem cells (ESCs) allows the production of transchromosomic mice, ESCs have unstable karyotypes and germline transmission is unreli...

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Bibliographic Details

Published in:	Stem Cell Reports
Main Authors:	Takashi Shinohara, Kanako Kazuki, Narumi Ogonuki, Hiroko Morimoto, Shogo Matoba, Kei Hiramatsu, Kazuhisa Honma, Teruhiko Suzuki, Takahiko Hara, Atsuo Ogura, Mitsuo Oshimura, Mito Kanatsu-Shinohara, Yasuhiro Kazuki
Format:	Article
Language:	English
Published:	Elsevier 2017-10-01
Subjects:	spermatogenesis karyotype artificial chromosome spermatogonia
Online Access:	http://www.sciencedirect.com/science/article/pii/S2213671117303697