Protocol to establish a genetically tractable synthetic symbiosis between Sodalis praecaptivus and grain weevils by insect egg microinjection

Summary: We present a protocol to establish a synthetic symbiosis between the mCherry-expressing Sodalis praecaptivus and the grain weevil host, Sitophilus zeamais. We describe steps to isolate grain weevil eggs, followed by microinjecting the bacterial symbiont into insect eggs using a modified Dro...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Yinghua Su, Ho-Chen Lin, Colin Dale
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Evolutionary Biology Microbiology Model Organisms
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723001144