DNA Shuffling Method for Generating Estrogen Receptor α and β Chimeras in Yeast
To facilitate our study of the molecular basis for the estrogen receptor (ER) subtype selectivity of novel ligands, we used DNA shuffling to construct chimeric ERs having ligand binding domains derived from both ERα and ERβ. The efficiency of chimera generation was low with traditional DNA shuffling...
| Published in: | BioTechniques |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-02-01
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| Online Access: | https://www.future-science.com/doi/10.2144/03342st02 |