Protocol for analyzing the function of the type VI secretion system of the oral symbiont Aggregatibacter aphrophilus in targeting pathobionts

Summary: Here, we present a protocol for evaluating type VI secretion system (T6SS)-dependent fitness of the oral symbiont A. aphrophilus using biofilm competition assays and metaproteomics. We describe steps for designing T6SS-specific mutants. We then detail procedures for using them in competitio...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Jan Oscarsson, Kai Bao, Akiko Shiratsuchi, Jonas Grossmann, Witold Wolski, Kyaw Min Aung, Mark Lindholm, Anders Johansson, Ferdousi Rahman Mowsumi, Sun Nyunt Wai, Georgios N. Belibasakis, Nagihan Bostanci
Format:	Article
Language:	English
Published:	Elsevier 2024-12-01
Subjects:	genomics microbiology molecular biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672400580X