TaqMan-quantitative PCR assays applied in Neospora caninum knock-outs generated through CRISPR-Cas9 allow to determine the copy numbers of integrated dihydrofolate reductase-thymidylate synthase drug selectable markers

As for many other organisms, CRISPR-Cas9 mediated genetic modification has gained increasing importance for the identification of vaccine candidates and drug targets in Neospora caninum, an apicomplexan parasite causing abortion in cattle and neuromuscular disease in dogs. A widely used approach for...

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Bibliographic Details

Published in:	Frontiers in Cellular and Infection Microbiology
Main Authors:	Laura Rico-San Román, Kai Pascal Alexander Hänggeli, Andrew Hemphill, Pilar Horcajo, Esther Collantes-Fernández, Luis Miguel Ortega-Mora, Ghalia Boubaker
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-06-01
Subjects:	duplex TaqMan-qPCR single TaqMan-qPCR DHFR-TS mdhfr-ts CRISPR-Cas9 off target effects
Online Access:	https://www.frontiersin.org/articles/10.3389/fcimb.2024.1419209/full