Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

Abstract Over the past few years, tools that make use of the Cas9 nuclease have led to many breakthroughs, including in the control of gene expression. The catalytically dead variant of Cas9 known as dCas9 can be guided by small RNAs to block transcription of target genes, in a strategy also known a...

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Bibliographic Details

Published in:	Molecular Systems Biology
Main Authors:	Antoine Vigouroux, Enno Oldewurtel, Lun Cui, David Bikard, Sven van Teeffelen
Format:	Article
Language:	English
Published:	Springer Nature 2018-03-01
Subjects:	CRISPR‐dCas9 CRISPRi gene‐expression noise peptidoglycan cell wall single‐cell
Online Access:	https://doi.org/10.15252/msb.20177899