Optimized Peptide–MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells

Peptide–MHC (pMHC) multimers have become the “gold standard” for the detection and isolation of antigen-specific T-cells but recent evidence shows that normal use of these reagents can miss fully functional T-cells that bear T-cell receptors (TCRs) with low affinity for cognate antigen. This issue i...

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Bibliographic Details

Published in:	Frontiers in Immunology
Main Authors:	Garry Dolton, Efthalia Zervoudi, Cristina Rius, Aaron Wall, Hannah L. Thomas, Anna Fuller, Lorraine Yeo, Mateusz Legut, Sophie Wheeler, Meriem Attaf, Dmitriy M. Chudakov, Ernest Choy, Mark Peakman, Andrew K. Sewell
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2018-06-01
Subjects:	peptide–MHC multimer tetramer dextramer autoimmune disease T-cell type 1 diabetes
Online Access:	https://www.frontiersin.org/article/10.3389/fimmu.2018.01378/full