Direct and accurate miRNA detection based on CRISPR/Cas13a-triggered exonuclease-iii-assisted colorimetric assay

Abstract The abnormally expressed microRNAs (miRNAs) serve as crucial indicators for disease diagnosis and are strongly associated with the progression of various diseases. The quantification of miRNAs is highly significant for the clinical diagnosis and treatment of various types of cancers. This s...

Full description

Bibliographic Details
Published in:	Journal of Analytical Science and Technology
Main Authors:	Yunxiao Li, Qiuxia Wang, Yali Wang
Format:	Article
Language:	English
Published:	SpringerOpen 2024-04-01
Subjects:	microRNAs (miRNAs) Exonuclease-iii (Exo-iii) Clustered regularly interspaced short palindromic repeats (CRISPR) Color reaction
Online Access:	https://doi.org/10.1186/s40543-024-00434-4

Similar Items

Exonuclease III assisted exponential amplification reaction (EXPAR) for specific miRNA-155 analysis during post-anesthetic nursing
by: Xuejun Wu, et al.
Published: (2024-06-01)

Bacterial exonuclease III expands its enzymatic activities on single-stranded DNA
by: Hao Wang, et al.
Published: (2024-07-01)

CRISPR/Cas12a Collateral Cleavage Activity for Sensitive 3′–5′ Exonuclease Assay
by: Jae Hoon Jeung, et al.
Published: (2023-10-01)

Application of the Streptococcus pyogenes CRISPR/Cas9 system in Lacticaseibacillus paracasei CGMCC4691
by: Sichang Fang, et al.
Published: (2025-09-01)

CRISPR/Cas9 revitalizes adoptive T-cell therapy for cancer immunotherapy
by: Sasan Ghaffari, et al.
Published: (2021-08-01)

Detection and Distribution of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in Campylobacter jejuni Isolates from Chicken Livers
by: Hung-Yueh Yeh, et al.
Published: (2024-04-01)

Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)
by: KOU Xiuying, ZHOU Baoqing, CHEN Ling, DAI Jingsha, ZHANG Jumei, WU Qingping
Published: (2023-04-01)

Applications of CRISPR-Cas-Based Genome Editing Approaches Against Human Cytomegalovirus Infection
by: Andra Zhang, et al.
Published: (2025-06-01)

Recent Advances in CRISPR/Cas System-Based Biosensors for the Detection of Foodborne Pathogenic Microorganisms
by: Sanlei Xie, et al.
Published: (2024-10-01)

Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms
by: DAI Yongjin, SUN Jing, ZHANG Moran, LIU Yujuan, CHEN Hongzhou, LU Yingjian
Published: (2024-11-01)

Plant genome editing using engineered nucleases and success of CRISPR/Cas9 system
by: Moon Sajid, et al.
Published: (2017-08-01)

Boosting CAR-T cell therapy with CRISPR technology
by: Liyanran Yan, et al.
Published: (2024-08-01)

Inconsistent treatments of the kinetics of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) impair assessment of its diagnostic potential
by: Juan G. Santiago
Published: (2022-01-01)

CRISPR/Cas9-mediated Targeted Mutagenesis of Inulin Biosynthesis in Rubber Dandelion
by: Menaka Ariyaratne, et al.
Published: (2023-11-01)

Is ignorance bliss? Milk from gene-edited cows and animal welfare considerations
by: Jill J. McCluskey, et al.

Progress and Perspective of CRISPR‐Cas9 Technology in Translational Medicine
by: Ruixuan Zheng, et al.
Published: (2023-09-01)

Rapid Generation of Long Noncoding RNA Knockout Mice Using CRISPR/Cas9 Technology
by: Nils R. Hansmeier, et al.
Published: (2019-01-01)

Exploring Advanced CRISPR Delivery Technologies for Therapeutic Genome Editing
by: Neda Rostami, et al.
Published: (2024-10-01)

Progress and challenges in applying CRISPR/Cas techniques to the genome editing of trees
by: Solme Pak, et al.
Published: (2022-01-01)

Exonuclease III Can Efficiently Cleave Linear Single-Stranded DNA: Reshaping Its Experimental Applications in Biosensors
by: Yi Shen, et al.
Published: (2023-05-01)

CRISPR-Cas-Integrated LAMP
by: Nazente Atçeken, et al.
Published: (2022-11-01)

Electrosprayed Alginate Nanoparticles as CRISPR Plasmid DNA Delivery Carrier: Preparation, Optimization, and Characterization
by: Batoul Alallam, et al.
Published: (2020-07-01)

CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review
by: Seungyeon Kang, et al.
Published: (2025-02-01)

Description of CRISPR-Cas9 development and its prospects in human papillomavirus-driven cancer treatment
by: Yuhao Wei, et al.
Published: (2022-11-01)

Anticipating On‐Target and Off‐Target Effects of CRISPR/Cas9 Genome Editing Via a Feedforward Neural Network Model
by: Pavithra Nagendran, et al.
Published: (2025-06-01)

Rapid Single-Tube Detection of Peach (Prunus persica)-Derived Ingredients by Recombinase-Aided Amplification Combined with Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-Associated Proteins (Cas)12a
by: WANG Lin, XING Ranran, YU Yaoxian, YI Qiuju, LU Siyuan, DENG Tingting, WANG Xu, CHEN Ying
Published: (2024-11-01)

CRISPR‐based environmental DNA detection for a rare endangered estuarine species
by: Raman P. Nagarajan, et al.
Published: (2024-01-01)

Ultrasensitive and colorimetric small extracellular vesicles (sEVs) analysis via dual-cycle signal tool
by: Linhong Ning, et al.
Published: (2023-01-01)

Application of engineered CRISPR/Cas12a variants with altered protospacer adjacent motif specificities for the detection of isoniazid resistance mutations in Mycobacterium tuberculosis
by: Peng Liu, et al.
Published: (2025-10-01)

Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis
by: Sheng Gao, et al.
Published: (2020-01-01)

Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review
by: Hiya Borah, et al.
Published: (2025-12-01)

A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach
by: Yu Chen, et al.
Published: (2021-10-01)

CRISPR–Cas systems and applications for crop bioengineering
by: Mireia Uranga, et al.
Published: (2024-10-01)

Based on CRISPR-Cas13a system, to establish a rapid visual detection method for avian influenza viruses
by: Zongshu Zhang, et al.
Published: (2024-01-01)

Crossing a CRISPR/Cas9 transgenic tomato plant with a wild-type plant yields diverse mutations in the F1 progeny
by: Yasuhiro Ito
Published: (2024-08-01)

Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges
by: Bashdar Mahmud Hussen, et al.
Published: (2023-07-01)

Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes
by: Xiaoping Li, et al.
Published: (2023-08-01)

Enzyme-Assisted Amplification and Copper Nanocluster Fluorescence Signal-Based Method for miRNA-122 Detection
by: Yang Qing, et al.
Published: (2023-08-01)

Ov-RPA–CRISPR/Cas12a assay for the detection of Opisthorchis viverrini infection in field-collected human feces
by: Orawan Phuphisut, et al.
Published: (2024-02-01)

CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen production
by: Michael A. Gomez, et al.
Published: (2023-03-01)