Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

Summary: Currently, the only approach for obtaining alveolar epithelial type I (AT1) cells is through the differentiation of cultured AT2 cells, which has several limitations. Here, we present a protocol to prepare AT1 cells directly from mouse lungs. We detail the steps for dissociating epithelial...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Lulu Huang, Amy Tang, Bojing Shao
Format:	Article
Language:	English
Published:	Elsevier 2024-12-01
Subjects:	cell biology cell culture cell isolation cell separation/fractionation mass cytometry
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672400649X