De novo peptide databases enable protein-based stable isotope probing of microbial communities with up to species-level resolution

Abstract Background Protein-based stable isotope probing (Protein-SIP) is a powerful approach that can directly link individual taxa to activity and substrate assimilation, elucidating metabolic pathways and trophic relationships within microbial communities. In Protein-SIP, peptides and correspondi...

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Bibliographic Details
Published in:Environmental Microbiome
Main Authors: Simon Klaes, Christian White, Lisa Alvarez-Cohen, Lorenz Adrian, Chang Ding
Format: Article
Language:English
Published: BMC 2025-08-01
Subjects:
Proteomics
Metaproteomics
De novo peptide sequencing
Microbiome
Microbiota
Microbiomics
Online Access:https://doi.org/10.1186/s40793-025-00767-6

Internet

https://doi.org/10.1186/s40793-025-00767-6

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