A comprehensive evaluation of a novel targeted-sequencing workflow for Mycobacterium species identification and anti-tuberculosis drug-resistance detection

BackgroundAlthough several World Health Organization–endorsed targeted next-generation sequencing (tNGS) assays exist for tuberculosis (TB) drug-resistance detection, their target selection and diagnostic accuracy vary widely. In this study, we developed a novel tNGS workflow (the TB Pro assay) and...

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Bibliographic Details

Published in:	Frontiers in Cellular and Infection Microbiology
Main Authors:	Xichao Ou, Shaojun Pei, Hongru Li, Zhonghua Qin, Richard Anthony, Jichun Wang, Zexuan Song, Ruida Xing, Lixia Zhang, Chong Teng, Hui Xia, Yang Zhou, Yuanyuan Song, Yang Zheng, Shengfen Wang, Bing Zhao, Yanlin Zhao
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-06-01
Subjects:	Mycobacterium tuberculosis non-tuberculous mycobacteriosis drug resistance multiplex PCR (polymerase chain reaction) targeted next generation sequencing (tNGS)
Online Access:	https://www.frontiersin.org/articles/10.3389/fcimb.2025.1584237/full