NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing

Abstract Background Current approaches to profile the single-cell transcriptomics of human pancreatic endocrine cells almost exclusively rely on freshly isolated islets. However, human islets are limited in availability. Furthermore, the extensive processing steps during islet isolation and subseque...

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Published in:	BMC Genomics
Main Authors:	Gengqiang Xie, Maria Pilar Toledo, Xue Hu, Hyo Jeong Yong, Pamela Sandoval Sanchez, Chengyang Liu, Ali Naji, Jerome Irianto, Yue J. Wang
Format:	Article
Language:	English
Published:	BMC 2024-04-01
Subjects:	Single-nucleus RNA-seq NKX2-2 Fluorescence-activated nuclei sorting (FANS) Frozen archival human pancreas Islets Endocrine population enrichment
Online Access:	https://doi.org/10.1186/s12864-024-10335-w