Hairpin loop to hairpin loop: a full-length assembly of the ASFV genome using Oxford Nanopore long-read sequencing

Short-read assembly of the African swine fever virus (ASFV) genome is challenging due to the presence of inverted terminal repeat (ITR) and hairpin loop sequences, which often cause ambiguity in contig reconstruction. In this study, we employed Oxford Nanopore long-read sequencing to assemble a full...

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Bibliographic Details

Published in:	Frontiers in Microbiology
Main Authors:	Poompat Phadphon, Chutima Sonthirod, Theeradej Thaweerattanasinp, Jeremy R. Shearman, Sonicha U-thoomporn, Janya Saenboonrueng, Asawin Wanitchang, Sithichoke Tangphatsornruang, Anan Jongkaewwattana, Wirulda Pootakham
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-08-01
Subjects:	hairpin loop MA-104 cells African swine fever virus ASFV adaptation
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2025.1615977/full