Efficient multi-allelic genome editing via CRISPR–Cas9 ribonucleoprotein-based delivery to Brassica napus mesophyll protoplasts

Canola (Brassica napus L.) is a valuable oilseed crop worldwide. However, trait improvement by breeding has been limited by its low genetic diversity and polyploid genetics. Whilst offering many potential benefits, the application of transgenic technology is challenged by the stringent and expensive...

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Bibliographic Details

Published in:	Frontiers in Plant Science
Main Authors:	Sareena Sahab, Fatima Runa, Mahilini Ponnampalam, Pippa T. Kay, Elizabeth Jaya, Katerina Viduka, Stephen Panter, Josquin Tibbits, Matthew J. Hayden
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-11-01
Subjects:	canola genome editing CRISPR Cas9 ribonucleoprotein protoplasts
Online Access:	https://www.frontiersin.org/articles/10.3389/fpls.2024.1397632/full