Improved image contrast in nonlinear light-sheet fluorescence microscopy using i $$^2$$ 2 PIE Pulse compression

Abstract Nonlinear microscopy has become an invaluable tool for biological imaging, offering high-resolution visualization of biological specimens. In this manuscript, we present the application of a spectral phase measurement technique, i $$^2$$ 2 PIE, to compress broad-bandwidth supercontinuum pul...

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Bibliographic Details
Published in:Scientific Reports
Main Authors: Imraan Badrodien, Pieter H. Neethling, Gurthwin W. Bosman
Format: Article
Language:English
Published: Nature Portfolio 2024-06-01
Subjects:
Online Access:https://doi.org/10.1038/s41598-024-63429-6