Donor Variability and Seeding Density Shape NK-Cell Proliferation and Surface Receptor Expression: Insights from an Integrated Phenotypic and Genetic Analysis

Natural killer (NK) cells are promising candidates for adoptive immunotherapy, but their clinical application requires standardized expansion protocols that yield functional cells in sufficient numbers. This study examined how initial seeding density and donor-intrinsic variability affect NK cell pr...

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Bibliographic Details

Published in:	Cells
Main Authors:	Neele Kusch, Jonathan Storm, Antonia Macioszek, Cornelius Knabbe, Barbara Kaltschmidt, Christian Kaltschmidt
Format:	Article
Language:	English
Published:	MDPI AG 2025-08-01
Subjects:	natural killer cells G-Rex culture system seeding density receptor expression donor variability flow cytometry
Online Access:	https://www.mdpi.com/2073-4409/14/16/1252