A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity

A continuous assay using internally quenched fluorescent peptides with the general sequence Abz-peptidyl-(Dnp)P-OH (Abz = ortho-aminobenzoic acid; Dnp = 2,4-dinitrophenyl) was optimized for the measurement of angiotensin I-converting enzyme (ACE) in human plasma and rat tissues. Abz-FRK(Dnp)P-OH, wh...

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Bibliographic Details

Published in:	Brazilian Journal of Medical and Biological Research
Main Authors:	M.F. Alves, M.C. Araujo, M.A. Juliano, E.M. Oliveira, J.E. Krieger, D.E. Casarini, L. Juliano, A.K. Carmona
Format:	Article
Language:	English
Published:	Associação Brasileira de Divulgação Científica 2005-06-01
Subjects:	Angiotensin-converting enzyme activity Fluorometric assay Rat tissue angiotensin- converting enzyme Human plasma angiotensin-converting enzyme
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600007