Improved circulating tumor DNA identification for detection of esophageal squamous cell carcinoma by enzymatic methyl sequencing and hybrid neural network

Abstract Detection of cancer at early stage can significantly improve the five-year survival rate of patients. Bisulfite-based methylation detection can cause DNA damage, especially in high GC content regions which was associated with the development of cancers. Loss of aberrant methylated CpG sites...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Jiangfeng Shen, Yuqi Ren, Ziyong Mao, Qiuxiang Hu, Yilong Wan, Jiangcun Du, Yanting Lai, Shengxiang Shao, Liuqing Zhang, Hao Wu, Jiaxi Li, Sheng Ju, Xin Tong, Jun Zhao, Lei Cao, Deyi Xiong, ChengCheng Xu, Jun-Chi Xu, Dong Jiang
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-09-01
Subjects:	Bisulfite-based methyl detection Enzyme-based methyl detection CfDNA CtDNA Differentially methylated regions CpG sites
Online Access:	https://doi.org/10.1038/s41598-025-18278-2