Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes

Summary: Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell f...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Irene Ganan-Gomez, Karen Clise-Dwyer, Simona Colla
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Subjects:	Cell Biology Cell culture Cell isolation Flow Cytometry/Mass Cytometry Cell separation/fractionation Cancer
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672200644X