Analytical validation of an error-corrected ultra-sensitive ctDNA next-generation sequencing assay

Plasma circulating tumor DNA (ctDNA) analysis has emerged as a minimally invasive means to perform molecular tumor typing. Here we developed a custom ultra-sensitive ctDNA next-generation sequencing assay using molecular barcoding technology and off-the-shelf reagents combined with bioinformatics to...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	Heidi Fettke, Jason A Steen, Edmond M Kwan, Patricia Bukczynska, Shivakumar Keerthikumar, David Goode, Maria Docanto, Nicole Ng, Luciano Martelotto, Christine Hauser, Melissa C Southey, Arun A Azad, Tu Nguyen-Dumont
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2020-08-01
Subjects:	cell-free DNA cfDNA circulating tumor DNA ctDNA liquid biopsy molecular barcoding
Online Access:	https://www.future-science.com/doi/10.2144/btn-2020-0045