Amplification of 4–9-kb Human Genomic DNA Flanking a Known Site Using a Panhandle PCR Variant
We present a method for the in vitro amplification of >6.0 kb of DNA flanking a known site. This is accomplished by ligating an oligonucleotide to create an inverted repeat of a portion of the known sequence, followed by single-primer polymerase chain reaction (PCR) amplifications. This method ge...
| Published in: | BioTechniques |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-07-01
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| Online Access: | https://www.future-science.com/doi/10.2144/97231rr01 |