Massively Parallel CRISPR-Cas9 Knockout Screening in Sheep Granulosa Cells for FSH Response Genes

Follicle-stimulating hormone (FSH) regulates ovarian follicle development through specific gene expression programs. Granulosa cells (GCs) are somatic cells surrounding the oocytes, secreting gonadotropins to regulate ovulation and promote follicular development. By analyzing the effects of differen...

Full description

Bibliographic Details

Published in:	Animals
Main Authors:	Zaixia Liu, Lingli Dai, Tianhao Sun, Yongbin Liu, Yanchun Bao, Mingjuan Gu, Shaoyin Fu, Xiaolong He, Caixia Shi, Yu Wang, Lili Guo, Le Zhou, Fengying Ma, Risu Na, Wenguang Zhang
Format:	Article
Language:	English
Published:	MDPI AG 2024-03-01
Subjects:	granulosa cells FSH massively parallel CRISPR-Cas9 library dose-dependent genes
Online Access:	https://www.mdpi.com/2076-2615/14/6/898