Peptide tags for labeling membrane proteins in live cells with multiple fluorophores

We describe a method to label specific membrane proteins with fluorophores for live imaging. Fusion proteins are generated that incorporate into their extracellular domains short peptide sequences (13–38 amino acids) recognized with high affinity and specificity by protein ligands, α-bungarotoxin (B...

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Bibliographic Details
Published in:BioTechniques
Main Authors: Corey M. McCann, Florence M. Bareyre, Jeff W. Lichtman, Joshua R. Sanes
Format: Article
Language:English
Published: Taylor & Francis Group 2005-06-01
Online Access:https://www.future-science.com/doi/10.2144/05386IT02