Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics

Summary: Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Junyi Zhang, Jakob Straehle, Kevin Joseph, Nicolas Neidert, Simon Behringer, Jonathan Göldner, Andreas Vlachos, Marco Prinz, Christian Fung, Jürgen Beck, Oliver Schnell, Dieter Henrik Heiland, Vidhya M. Ravi
Format:	Article
Language:	English
Published:	Elsevier 2023-09-01
Subjects:	Cell Biology Cell Isolation Single Cell Flow Cytometry/Mass Cytometry Cancer Sequencing
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723003507