Accurate Quantification of Escherichia coli O157 in Grape Juice Using Hydrogel-Based Digital Polymerase Chain Reaction

In this study, we developed a direct hydrogel digital polymerase chain reaction (Direct-hdPCR) technique for the detection of Escherichia coli O157 in grape juice. In this method, samples were directly mixed with a hydrogel PCR system, followed by release of bacterial DNA through thermal lysis, and...

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Bibliographic Details

Published in:	Shipin Kexue
Main Author:	YI Zihan, LIN Xingyu
Format:	Article
Language:	English
Published:	China Food Publishing Company 2025-08-01
Subjects:	foodborne pathogens; escherichia coli o157; hydrogel; digital polymerase chain reaction; fluorescence detection
Online Access:	https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-15-001.pdf