Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines

Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful...

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Bibliographic Details

Published in:	Stem Cell Research
Main Authors:	C.H. Cole Sims, Matias I. Autio, Adriana Buskin, Christine Cheung, Rakesh Heer, Roger S.Y. Foo, Xin Wang
Format:	Article
Language:	English
Published:	Elsevier 2021-04-01
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506121000842