Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota

Abstract Background Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples. Metabarcoding using short-read sequencers, such as Illumina platf...

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Bibliographic Details
Published in:Environmental Microbiome
Main Authors: Jana Mittelstrass, Renate Heinzelmann, René Eschen, Martin Hartmann, Quirin Kupper, Salome Schneider, Simone Prospero, Iva Franić
Format: Article
Language:English
Published: BMC 2025-05-01
Subjects:
High-throughput sequencing
Amplicon sequencing
Read length
MinION
ITS
Endophytes
Online Access:https://doi.org/10.1186/s40793-025-00712-7

Internet

https://doi.org/10.1186/s40793-025-00712-7

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