Action of CMG with strand-specific DNA blocks supports an internal unwinding mode for the eukaryotic replicative helicase

Replicative helicases are ring-shaped hexamers that encircle DNA for duplex unwinding. The currently accepted view of hexameric helicase function is by steric exclusion, where the helicase encircles one DNA strand and excludes the other, acting as a wedge with an external DNA unwinding point during...

Full description

Bibliographic Details
Published in:	eLife
Main Authors:	Lance Langston, Mike O’Donnell
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2017-03-01
Subjects:	replicative helicase DNA replication helicase mechanism
Online Access:	https://elifesciences.org/articles/23449

Similar Items

An explanation for origin unwinding in eukaryotes
by: Lance D Langston, et al.
Published: (2019-07-01)

The replicative CMG helicase: the ideal target for cancer therapy
by: W. Henderson, et al.
Published: (2020-12-01)

CMG helicase disassembly is controlled by replication fork DNA, replisome components and a ubiquitin threshold
by: Tom D Deegan, et al.
Published: (2020-08-01)

Viral hijacking of a replicative helicase loader and its implications for helicase loading control and phage replication
by: Iris V Hood, et al.
Published: (2016-05-01)

Mechanisms of opening and closing of the bacterial replicative helicase
by: Jillian Chase, et al.
Published: (2018-12-01)

Preparing to unwind
by: Jin Chuan Zhou, et al.
Published: (2014-04-01)

Two opposite abilities of the infectious bronchitis virus helicase Nsp13: separating the duplex and promoting the annealing of single-stranded nucleic acid
by: Chengcheng Wu, et al.
Published: (2025-03-01)

Primosomal protein PriC rescues replication initiation stress by bypassing the DnaA-DnaB interaction step for DnaB helicase loading at oriC
by: Ryusei Yoshida, et al.
Published: (2025-05-01)

A conserved MCM single-stranded DNA binding element is essential for replication initiation
by: Clifford A Froelich, et al.
Published: (2014-04-01)

Identification of 1600 replication origins in S. cerevisiae
by: Eric J Foss, et al.
Published: (2024-02-01)

RNA Helicase A Regulates the Replication of RNA Viruses
by: Rui-Zhu Shi, et al.
Published: (2021-02-01)

RecQ helicases and genome stability: lessons from model organisms and human disease
by: L Bjergbaek, et al.
Published: (2002-08-01)

Joint Efforts of Replicative Helicase and SSB Ensure Inherent Replicative Tolerance of G‐Quadruplex
by: Lijuan Guo, et al.
Published: (2024-03-01)

A back-up mechanism for replication
by: Godefroid Charbon, et al.
Published: (2025-05-01)

Ufd1-Npl4 Recruit Cdc48 for Disassembly of Ubiquitylated CMG Helicase at the End of Chromosome Replication
by: Marija Maric, et al.
Published: (2017-03-01)

DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication
by: Chen-Yu Lo, et al.
Published: (2021-08-01)

A Decade of Discovery—Eukaryotic Replisome Disassembly at Replication Termination
by: Rebecca M. Jones, et al.
Published: (2024-03-01)

The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader
by: Neha Puri, et al.
Published: (2021-05-01)

Inteins at Eleven Distinct Insertion Sites in Archaeal Helicase Subunit MCM Exhibit Varied Architectures and Activity Levels Across Archaeal Groups
by: Danielle Arsenault, et al.
Published: (2025-08-01)

Sequence-dependent base pair stepping dynamics in XPD helicase unwinding
by: Zhi Qi, et al.
Published: (2013-05-01)

Managing Single-Stranded DNA during Replication Stress in Fission Yeast
by: Sarah A. Sabatinos, et al.
Published: (2015-09-01)

A helicase-tethered ORC flip enables bidirectional helicase loading
by: Shalini Gupta, et al.
Published: (2021-12-01)

Helicases at Work: The Importance of Nucleic Acids Unwinding Under Cold Stress
by: Theetha L. Pavankumar, et al.
Published: (2024-11-01)

Methotrexate, an anti-inflammatory drug, inhibits Hepatitis E viral replication
by: Akash Kumar, et al.
Published: (2023-12-01)

Dengue Virus NS4b N-Terminus Disordered Region Interacts with NS3 Helicase C-Terminal Subdomain to Enhance Helicase Activity
by: Satyamurthy Kundharapu, et al.
Published: (2022-08-01)

A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding
by: Kanokwan Champasa, et al.
Published: (2019-08-01)

Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile
by: Erika van Eijk, et al.
Published: (2016-01-01)

Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication
by: FuJung Chang, et al.
Published: (2015-08-01)

Analysis of the PcrA-RNA polymerase complex reveals a helicase interaction motif and a role for PcrA/UvrD helicase in the suppression of R-loops
by: Inigo Urrutia-Irazabal, et al.
Published: (2021-07-01)

Werner helicase interacting protein 1 contributes to G-quadruplex processing in human cells
by: Lili Hegedus, et al.
Published: (2024-07-01)

Nucleosomes influence multiple steps during replication initiation
by: Ishara F Azmi, et al.
Published: (2017-03-01)

The Vaccinia Virus DNA Helicase Structure from Combined Single-Particle Cryo-Electron Microscopy and AlphaFold2 Prediction
by: Stephanie Hutin, et al.
Published: (2022-10-01)

The emerging role of DEAD/H-box helicases in hepatitis B virus infection
by: Hongjuan You, et al.
Published: (2022-11-01)

Interaction and assembly of the DNA replication core proteins of Kaposi’s sarcoma-associated herpesvirus
by: Lee-Wen Chen, et al.
Published: (2023-12-01)

Unwinding new therapeutic opportunities in rhabdomyosarcoma: the role of RNA helicase DDX5
by: Valeria Bianconi, et al.
Published: (2025-08-01)

Inhibitory effects of anthracyclines on partially purified 5′–3′ DNA helicase of Plasmodium falciparum
by: Pongruj Rattaprasert, et al.
Published: (2022-07-01)

Oxysterol binding protein (OSBP) contributes to hepatitis E virus replication
by: Shaoli Lin, et al.
Published: (2024-07-01)

Specific Monitoring the DNA Helicase Function via Anchor‐Embedded DNA Probe
by: Keni Ning, et al.
Published: (2025-02-01)

Interplay between chromosomal architecture and termination of DNA replication in bacteria
by: Daniel J. Goodall, et al.
Published: (2023-06-01)

DEAD-box RNA helicase 10 inhibits porcine circovirus type 3 replication by interacting with the viral capsid protein and activating interferon responses
by: Jianwei Zhou, et al.
Published: (2025-06-01)