Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

Summary: This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible genomic label. By extracting DNA from a complete adipose tissue depot stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Ucp1-CreERT2 x ROSA26-tdRFP), the n...

Full description

Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Caroline Moser, Leon G. Straub, Yael Rachamin, Christian Wolfrum
Format:	Article
Language:	English
Published:	Elsevier 2021-09-01
Subjects:	Cell culture Cell isolation Cell-based Assays Cell separation/fractionation Metabolism Molecular Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721004676