Evaluation of DNA extraction methods and direct PCR in metabarcoding of mock and marine bacterial communities

Recent advances in new molecular biology methods and next-generation sequencing (NGS) technologies have revolutionized metabarcoding studies investigating complex microbial communities from various environments. The inevitable first step in sample preparation is DNA extraction which introduces its o...

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Bibliographic Details

Published in:	Frontiers in Microbiology
Main Authors:	Iva Stojan, Željka Trumbić, Ivana Lepen Pleić, Danijela Šantić
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-04-01
Subjects:	DNA extraction mock community marine bacteria 16S rRNA direct PCR DNA metabarcoding
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1151907/full