Interkit Reproducibility of the Indirect Immunofluorescence Assay on HEp-2 Cells Depends on the Immunofluorescence Reactivity Intensity and Pattern

IntroductionThe indirect immunofluorescence assay on HEp-2 cells (HEp-2/IFA) is used worldwide for screening for autoantibodies to cellular antigens. Cell culture and fixation methods influence the cell distribution of autoantigens and the preservation of epitopes. Therefore, discrepancy of results...

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Bibliographic Details

Published in:	Frontiers in Immunology
Main Authors:	Mônica Jesus Silva, Alessandra Dellavance, Danielle Cristiane Baldo, Silvia Helena Rodrigues, Marcelle Grecco, Monica Simon Prado, Renan Agustinelli, Luís Eduardo Coelho Andrade
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022-01-01
Subjects:	autoantibody antinuclear antibodies immunofluorescence HEp-2 cells autoimmune diseases
Online Access:	https://www.frontiersin.org/articles/10.3389/fimmu.2021.798322/full