Structure-guided steric hindrance engineering of Bacillus badius phenylalanine dehydrogenase for efficient l-homophenylalanine synthesis

Abstract Background Direct reductive amination of prochiral 2-oxo-4-phenylbutyric acid (2-OPBA) catalyzed by phenylalanine dehydrogenase (PheDH) is highly attractive in the synthesis of the pharmaceutical chiral building block l-homophenylalanine (l-HPA) given that its sole expense is ammonia and th...

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發表在:	Biotechnology for Biofuels
Main Authors:	Tao Wu, Xiaoqing Mu, Yuyan Xue, Yan Xu, Yao Nie
格式:	Article
語言:	英语
出版:	BMC 2021-10-01
主題:	l-Homophenylalanine Phenylalanine dehydrogenase Steric hindrance Enzyme engineering Catalytic efficiency Reductive amination
在線閱讀:	https://doi.org/10.1186/s13068-021-02055-0