Production of functional CD19 CAR T cells under hypoxic manufacturing conditions

IntroductionChronic lymphocytic leukemia (CLL) has proven difficult to treat with chimeric antigen receptor (CAR) T cell therapy. CLL cells can negatively alter T cell fitness and induce a pseudohypoxic state. We hypothesized that production of CAR T cells under restricted oxygen conditions resembli...

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Bibliographic Details

Published in:	Frontiers in Immunology
Main Authors:	Isabella Micallef Nilsson, Thomas Poiret, Jinhye Ryu, Mohammadali Mohammadpour, Johan Henriksson, Anders Österborg, Jonas Mattsson, Anna Schurich, Isabelle Magalhaes
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-10-01
Subjects:	CAR T cells hypoxia CLL (chronic lymphocytic leukemia) tumor microenvironment mitochondria
Online Access:	https://www.frontiersin.org/articles/10.3389/fimmu.2025.1675786/full