Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress

Summary: Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We desc...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Hyun Young Kim, Wonseok Lee, Xiao Liu, Haeum Jang, Sadatsugu Sakane, Raquel Carvalho-Gontijo Weber, Karin Diggle, Samuel A. Kerk, Christian M. Metallo, Tatiana Kisseleva, David A. Brenner
Format:	Article
Language:	English
Published:	Elsevier 2024-06-01
Subjects:	cell isolation metabolism organoid
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724002764