Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling

Highly multiplexed ddPCR for sensitive nucleic acid quantitation remains challenging due to limited fluorescence channels. Here the authors report quantitative amplicon sequencing (QASeq), a PCR-based molecular barcoding NGS approach which is compatible with high multiplexing.

Bibliographic Details

Published in:	Nature Communications
Main Authors:	Lucia Ruojia Wu, Peng Dai, Michael Xiangjiang Wang, Sherry Xi Chen, Evan N. Cohen, Gitanjali Jayachandran, Jinny Xuemeng Zhang, Angela V. Serrano, Nina Guanyi Xie, Naoto T. Ueno, James M. Reuben, Carlos H. Barcenas, David Yu Zhang
Format:	Article
Language:	English
Published:	Nature Portfolio 2022-04-01
Online Access:	https://doi.org/10.1038/s41467-022-29487-y